Device

Part:BBa_K2023014

Designed by: Célia Chenebault   Group: iGEM16_Ionis_Paris   (2016-10-14)


GFP coding device with Pr

This part allowed us to start the CelloCad side project.
In order to compare the RPU (Relative Promoter Unit) of the Pr promoter with the reference promoter BBa_J23101 we designed this part which is composed of Pr promoter (BBa_I723018), the RBS Elowitz 1999 (BBa_B0034), the GFP (BBa_E0040) and a double terminator (BBa_B0015).
To learn more about the CelloCad project, visit our website: http://2016.igem.org/Team:Ionis_Paris/Measurement

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1080


[edit]
Categories
//function/reporter/fluorescence
Parameters
emission511
excitation501
proteinGFP